Massive outsider Diore Lia is out of the Investec Derby at Epsom.
Trainer John Jenkins was forced to withdraw the filly from the premier Classic after she pulled a muscle.
The daughter of Yeats had been a controversial runner in the Derby after she was well beaten on two racecourse starts this season.
She was initially due to have been partnered by 7lb claimer Gina Mangan, but the British Horseracing Authority blocked her from riding due to a lack of experience.
Paddy Pilley was instead set to ride before Diore Lia's enforced absence.
Owner Mary Todd's brother Richard Aylward bred the horse and had been keen to generate publicity for Great Ormond Street Children's Hospital by running the filly on the biggest stage of Flat racing.